如何得到更多的細(xì)菌DNA？How to achieve a better bacterial DNA yield?

A) 多數(shù)情況下是因?yàn)闃悠返臄?shù)量太少。 細(xì)菌量一般在1億個(gè)細(xì)菌左右(1ml 1OD600)；B) 細(xì)胞未充分裂解; C) 增加溶菌酶的量; D) 延長(zhǎng)裂解時(shí)間；E) 可適當(dāng)減少洗脫液的體積。

A) Typically it was due to starting material too low, using 1 ml of 1 OD600; B) Bacterial cells were not completely lysed; C) Increase lysozyme; D) Increase lysis time; E) Reduce the volume of the elution buffer.