如何提高瓊脂糖膠DNA回收量？How to improve DNA recovery from agarose gel?

如何提高瓊脂糖膠DNA回收量？





A) DNA起始量較少; B) 短波長UV照射時間太長; C) 溶膠時間不夠; D) 對小于200bp DNA片段，可增加與磁珠的接觸時間或適當(dāng)減少溶膠液的體積。

A) Not enough DNA to start with; B) UV radiation too long; C) Gel was not completely dissolved.

D) If DNA fragment <200 bp, you need add longer time to incubate with magnetic beads, or reduce GE10 volume, add GE20.