為什么質(zhì)粒DNA中有多條帶？How to avoid multiple plasmid bands in the gel?

為什么質(zhì)粒DNA中有多條帶？

A) 可能是DNA提取過(guò)程中混合太劇烈; B) 加入PB20處理時(shí)間太長(zhǎng)了。

A) After addition of PB20, mix too vigorously; B) wait too long after adding PB20; C) Cells were vigorous growth.